The Transcriptional Activity of Neurog3 Affects Migration and Differentiation of Ectopic Endocrine Cells in Chicken Endoderm
Author
Summary, in English
Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than differentiate. In contrast, reducing the transcriptional activity of Neurog3 by deleting parts of the activation domain, by fusing Neurog3 to the engrailed repressor domain, or co-electroporating with Hdac1 greatly increased the proportion of glucagon expressing cells. Developmental Dynamics 239:1950-1966, 2010. (C) 2010 Wiley-Liss, Inc.
Department/s
Publishing year
2010
Language
English
Pages
1950-1966
Publication/Series
Developmental Dynamics
Volume
239
Issue
7
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Cell and Molecular Biology
Keywords
- endocrine competence
- glucagon
- differentiation
- pancreas
- fluorescence
- whole-mount
- in ovo electroporation
- neurogenin 3
- Neurog3
- Ngn3
Status
Published
ISBN/ISSN/Other
- ISSN: 1097-0177