Integrated selective enrichment target - a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases

Summary, in English

The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.

• Author: Simon Ekström; Lars Wallman; Johan Malm; Charlotte Becker; Hans Lilja; Thomas Laurell; György Marko-Varga
• Department/s: Department of Biomedical Engineering; Clinical Chemistry, Malmö; Centre for Analysis and Synthesis
• Publishing year: 2004
• Language: English
• Pages: 3769-3777
• Publication/Series: Electrophoresis
• Volume: 25
• Issue: 21-22
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15565686&query_hl=15&itool=pubmed_docsum; http://dx.doi.org/10.1002/elps.200406094
• Document type: Journal article
• Publisher: John Wiley & Sons Inc.
• Topic: Medicinal Chemistry
• Status: Published
• Research group: Clinical Chemistry, Malmö
• ISBN/ISSN/Other: ISSN: 0173-0835