Use of synchrotron-radiation-based FTIR imaging for characterizing changes in cell contents

Summary, in English

FTIR imaging of individual cells is still limited by the low signal-to-noise ratio obtained from analysis of such weakly absorbing organic matter when using a Globar IR source. In this study, we used FTIR imaging with a synchrotron radiation source and a focal plane array detector to determine changes in the cellular contents of cryofixed cells after culture for 48 h on Si3N4 substrate. Several spectral differences were observed for cells deprived of glucose compared with control cells: a lower amide I-to-amide II ratio (P < 0.01); a different secondary structure profile of proteins (obtained from amide I spectral region curve fitting), with a significant increase in non-ordered structure components (P < 0.01); and a higher nu(C = C-H)/nu (as)(CH3) absorption ratio (P < 0.01), suggesting increased unsaturation of fatty acyl chains. Therefore, our study has shown that FTIR imaging with a synchrotron radiation source enables determination of several spectral changes of individual cells between two experimental conditions, which thus opens the way to cell biology studies with this vibrational spectroscopy technique.

• Author: Seydou Yao; Michel Moenner; Anders Engdahl; Cyril Petibois
• Department/s: MAX IV Laboratory
• Publishing year: 2012
• Language: English
• Pages: 1311-1316
• Publication/Series: Analytical and Bioanalytical Chemistry
• Volume: 404
• Issue: 5
• Links: http://dx.doi.org/10.1007/s00216-012-6223-0
• Document type: Journal article
• Publisher: Springer
• Topic: Analytical Chemistry
• Keywords: Bioanalytical methods; Spectroscopy/instrumentation; IR; spectroscopy/Raman spectroscopy; Imaging; Synchrotron radiation
• Status: Published
• ISBN/ISSN/Other: ISSN: 1618-2642