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Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells

Author

  • Claire Hebrard
  • Emeline Cros-Perrial
  • Anders Ranegaard Clausen
  • Charles Dumontet
  • Jure Piskur
  • Lars Petter Jordheim

Summary, in English

Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.

Publishing year

2009

Language

English

Pages

1068-1075

Publication/Series

Nucleosides, Nucleotides & Nucleic Acids

Volume

28

Issue

11-12

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Biological Sciences

Keywords

  • bacteria
  • suicide gene
  • gene therapy
  • Deoxyribonucleoside kinases
  • gemcitabine
  • cancer
  • resistance

Status

Published

ISBN/ISSN/Other

  • ISSN: 1525-7770