Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells
Author
Summary, in English
Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.
Department/s
Publishing year
2009
Language
English
Pages
1068-1075
Publication/Series
Nucleosides, Nucleotides & Nucleic Acids
Volume
28
Issue
11-12
Document type
Journal article
Publisher
Taylor & Francis
Topic
- Biological Sciences
Keywords
- bacteria
- suicide gene
- gene therapy
- Deoxyribonucleoside kinases
- gemcitabine
- cancer
- resistance
Status
Published
ISBN/ISSN/Other
- ISSN: 1525-7770