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Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza

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Author

Summary, in English

Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.

Publishing year

2007

Language

English

Pages

644-647

Publication/Series

Molecular Ecology Notes

Volume

7

Issue

4

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Ecology

Keywords

  • narrowed-leaved marsh orchid
  • microsatellites
  • cross-species amplification
  • Dactylorhiza
  • polymorphism
  • polyploidy

Status

Published

ISBN/ISSN/Other

  • ISSN: 1471-8278