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Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Author

Summary, in English

We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.

Publishing year

2007

Language

English

Pages

1101-1113

Publication/Series

Journal of Proteome Research

Volume

6

Issue

3

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Immunology in the medical area

Keywords

  • precursor-ion
  • reagents
  • mass spectrometry
  • isotope labeling
  • HPLC
  • parent-ion

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-3893