Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis.
Author
Summary, in English
Extensive studies of mice deficient in one or several cytokine receptors have failed to support an indispensable role of cytokines in development of multiple blood cell lineages. Whereas B1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL-7, IL-7R{alpha}, common cytokine receptor gamma chain, or flt3 ligand (FL), we report here that adult mice double deficient in IL-7R{alpha} and FL completely lack visible LNs, conventional IgM+ B cells, IgA+ plasma cells, and B1 cells, and consequently produce no Igs. All stages of committed B cell progenitors are undetectable in FL-/- x IL-7R{alpha}-/- BM that also lacks expression of the B cell commitment factor Pax5 and its direct target genes. Furthermore, in contrast to IL-7R{alpha}-/- mice, FL-/- x IL-7R{alpha}-/- mice also lack mature B cells and detectable committed B cell progenitors during fetal development. Thus, signaling through the cytokine tyrosine kinase receptor flt3 and IL-7R{alpha} are indispensable for fetal and adult B cell development.
Department/s
- Stem Cell Center
- Immunology
- Neurology, Lund
Publishing year
2003
Language
English
Pages
1495-1506
Publication/Series
Journal of Experimental Medicine
Volume
198
Issue
10
Full text
- Available as PDF - 276 kB
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Document type
Journal article
Publisher
Rockefeller University Press
Topic
- Immunology in the medical area
- Cell and Molecular Biology
- Endocrinology and Diabetes
Keywords
- IL-7 receptor
- Flt3 ligand
- B1 cells
- Pax5
- lymphopoiesis
Status
Published
Research group
- Immunology
ISBN/ISSN/Other
- ISSN: 1540-9538