Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli
Author
Editor
- Marie-Noelle Pons
- Jan Van Impe
Summary, in English
A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.
Department/s
Publishing year
2004
Language
English
Pages
73-79
Publication/Series
Computer Applications in Biotechnology 2004
Document type
Conference paper
Publisher
International Federation of Automatic Control
Topic
- Control Engineering
- Industrial Biotechnology
Keywords
- temperature control
- Escherichia coli
- Fermentation process
- feeding strategy
Status
Published
ISBN/ISSN/Other
- ISBN: 0-08-044251-X