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Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli

Author

Editor

  • Marie-Noelle Pons
  • Jan Van Impe

Summary, in English

A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.

Publishing year

2004

Language

English

Pages

73-79

Publication/Series

Computer Applications in Biotechnology 2004

Document type

Conference paper

Publisher

International Federation of Automatic Control

Topic

  • Control Engineering
  • Industrial Biotechnology

Keywords

  • temperature control
  • Escherichia coli
  • Fermentation process
  • feeding strategy

Status

Published

ISBN/ISSN/Other

  • ISBN: 0-08-044251-X