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Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

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Author

Summary, in English

A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.

Publishing year

2002

Language

English

Pages

349-354

Publication/Series

Mycological Research

Volume

106

Issue

3

Document type

Journal article

Publisher

Cambridge University Press

Topic

  • Biological Sciences

Status

Published

Research group

  • Microbial Ecology

ISBN/ISSN/Other

  • ISSN: 0953-7562