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Estimating the variation in S phase duration from flow cytometric histograms.

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Author

Summary, in English

A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data.

Publishing year

2008

Language

English

Pages

40-49

Publication/Series

Mathematical Biosciences

Volume

213

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Cancer and Oncology

Keywords

  • Branching process
  • Cell cycle progression
  • S phase duration
  • Flow cytometry

Status

Published

ISBN/ISSN/Other

  • ISSN: 0025-5564