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Charges drive selection of specific antibodies by phage display.

På svenska

Author

  • Helena Persson
  • Jonas Persson
  • Lena Danielsson
  • Mats Ohlin

Summary, in English

Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection. Implications of and possible routes to minimize this effect are discussed.

Publishing year

2010

Language

English

Pages

24-30

Publication/Series

Journal of Immunological Methods

Volume

353

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1872-7905