Proteomics-protein profiling technology: the trend towards a microfabricated toolbox concept
Author
Summary, in English
The use of mono-crystalline silicon microstructures for high-speed and high sensitivity protein mapping and protein identification is presented as an important contribution within the field of proteomics. The various approaches for handling minute amounts of protein sample are described. Piezoelectric dispensing units developed in-house are being used for sample-handling and the generation of 60-pl droplets, interfacing with high-density microchip MALDI-TOF MS target plates having an array density of 4000 nanovials/square inch. Examples of applications to microtechnology, and future trends in our research are outlined. (C) 2001 Elsevier Science B.V. All rights reserved.
Department/s
Publishing year
2001
Language
English
Pages
225-231
Publication/Series
Trac. Trends in Analytical Chemistry
Volume
20
Issue
5
Document type
Journal article
Publisher
Elsevier
Topic
- Medical Engineering
Keywords
- proteomics
- microtechnology
- mass spectrometry
- piezoelectric
- dispensing
- nanovial
- FLIGHT MASS-SPECTROMETRY
- SAMPLE PREPARATION
- LASER
- MICRODISSECTION
- CHROMATOGRAPHY
- SEPARATIONS
- SYSTEMS
Status
Published
ISBN/ISSN/Other
- ISSN: 0165-9936