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Mannanase Man26A from Cellulomonas fimi has a mannan-binding module

Author

  • Dominik Stoll
  • Alisdair Boraston
  • Henrik Stålbrand
  • Bradley W McLean
  • Douglas G Kilburn
  • R Anthony J Warren

Summary, in English

A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble mannans. Man26Abm does not bind to cellulose, chitin or xylan. The Kd for binding of Man26Abm to locust bean gum (LBG) is ~0.2 μM. Man26A is the first mannanase reported to contain a mannan-binding module.

Publishing year

2000

Language

English

Pages

265-269

Publication/Series

FEMS Microbiology Letters

Volume

183

Issue

2

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Biological Sciences

Keywords

  • Cellulomonas fimi
  • Mannanase
  • Mannan-binding module
  • Affinity gel electrophoresis

Status

Published

ISBN/ISSN/Other

  • ISSN: 1574-6968