Degradation of Pyrimidines in Saccharomyces Kluyveri: Transamination of beta-Alanine.
Author
Summary, in English
Beta-alanine is an intermediate in the reductive degradation of uracil. Recently we have identified and characterized the Saccharomyces kluyveri PYD4 gene and the corresponding enzyme beta -alanine aminotransferase ((Sk)Pyd4p), highly homologous to eukaryotic gamma-aminobutyrate aminotransferase (GABA-AT). S. kluyveri has two aminotransferases, GABA aminotransferase ((Sk)Uga1p) with 80% and (Sk)Pyd4p with 55% identity to S. cerevisiae GABA-AT. (Sk)Pyd4p is a typical pyridoxal phosphate-dependent aminotransferase, specific for alpha -ketoglutarate (alpha KG), beta -alanine (BAL) and gamma -aminobutyrate (GABA), showing a ping-pong kinetic mechanism involving two half-reactions and substrate inhibition. (Sk)Uga1p accepts only alpha KG and GABA but not BAL, thus only (Sk)Pydy4p belongs to the uracil degradative pathway.
Department/s
Publishing year
2008
Language
English
Pages
794-799
Publication/Series
Nucleosides, Nucleotides & Nucleic Acids
Volume
27
Issue
6
Document type
Journal article
Publisher
Taylor & Francis
Topic
- Biological Sciences
Keywords
- Saccharomyces kluyveri
- Aminotransferase
- gene duplication
- γ-aminobutyrate
- β-alanine
Status
Published
ISBN/ISSN/Other
- ISSN: 1525-7770