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Degradation of Pyrimidines in Saccharomyces Kluyveri: Transamination of beta-Alanine.

Author

  • K D Schnackerz
  • Gorm Andersen
  • D Dobritzsch
  • Jure Piskur

Summary, in English

Beta-alanine is an intermediate in the reductive degradation of uracil. Recently we have identified and characterized the Saccharomyces kluyveri PYD4 gene and the corresponding enzyme beta -alanine aminotransferase ((Sk)Pyd4p), highly homologous to eukaryotic gamma-aminobutyrate aminotransferase (GABA-AT). S. kluyveri has two aminotransferases, GABA aminotransferase ((Sk)Uga1p) with 80% and (Sk)Pyd4p with 55% identity to S. cerevisiae GABA-AT. (Sk)Pyd4p is a typical pyridoxal phosphate-dependent aminotransferase, specific for alpha -ketoglutarate (alpha KG), beta -alanine (BAL) and gamma -aminobutyrate (GABA), showing a ping-pong kinetic mechanism involving two half-reactions and substrate inhibition. (Sk)Uga1p accepts only alpha KG and GABA but not BAL, thus only (Sk)Pydy4p belongs to the uracil degradative pathway.

Publishing year

2008

Language

English

Pages

794-799

Publication/Series

Nucleosides, Nucleotides & Nucleic Acids

Volume

27

Issue

6

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Biological Sciences

Keywords

  • Saccharomyces kluyveri
  • Aminotransferase
  • gene duplication
  • γ-aminobutyrate
  • β-alanine

Status

Published

ISBN/ISSN/Other

  • ISSN: 1525-7770