Gene expression profiling of differentiating embryonic stem cells expressing dominant negative fibroblast growth factor receptor 2.
Author
Summary, in English
Embryonic stein (ES) cells are derived from the inner cell mass of the blastocyst and can be cultured as three-dimensional embryoid bodies (EBs) in which embryonic pregastrulation stages are faithfully mimicked. Fibroblast growth factor receptors (mainly FGFR2) are involved in the first differentiation events during early mammalian embryogenesis. It has been demonstrated that the presence of FGFR2 is a prerequisite for laminin-111 and collagen type IV synthesis and subsequently basement membrane formation in EBs. To identify genes that are influenced by FGFR signalling, we performed global gene expression profiling of differentiating EBs expressing dominant negative FGFR2 (dnFGFR2), acquiring an extensive catalogue of down- and up-regulated genes. We show a strong down-regulation of endodermal and basement membrane related genes, which strengthen the view that the FGFR signalling pathway is a main stimulator of basement membrane synthesis in EBs. We further present down-regulation of genes previously not linked to FGFR signalling, and in addition an active transcription of some mesodermal related genes in differentiating dnFGFR2 EBs.
Department/s
- Åke Oldberg´s group
- Muscle Biology
- Department of Experimental Medical Science
Publishing year
2007
Language
English
Pages
197-205
Publication/Series
Matrix Biology
Volume
26
Full text
- Available as PDF - 345 kB
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Links
Document type
Journal article
Publisher
Elsevier
Topic
- Cell and Molecular Biology
Keywords
- ES cell differentiation
- Basement membrane
- Gene expression
- FGFR signalling
- Microarray
- Embryoid bodies
Status
Published
Research group
- Åke Oldberg´s group
- Muscle Biology
ISBN/ISSN/Other
- ISSN: 1569-1802