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Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

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Author

Summary, in English

A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

Publishing year

2006

Language

English

Pages

3147-3153

Publication/Series

Measurement Science & Technology

Volume

17

Issue

12

Document type

Journal article

Publisher

IOP Publishing

Topic

  • Analytical Chemistry
  • Medical Engineering

Keywords

  • piezodispensing
  • capillary force
  • microfluidics
  • MALDI
  • mass spectrometry
  • microchip
  • robotic
  • MALDI-TOF MS
  • automation
  • protein analysis
  • proteomics
  • solid phase extraction

Status

Published

ISBN/ISSN/Other

  • ISSN: 0957-0233