Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus
Author
Summary, in English
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
Department/s
Publishing year
1994
Language
English
Pages
87-91
Publication/Series
FEMS Microbiology Letters
Volume
121
Issue
1
Links
Document type
Journal article
Publisher
Oxford University Press
Topic
- Biological Sciences
Keywords
- Amino acid respiration
- Aminooxyacetate
- [14C]alanine
- Ectomycorrhizal fungus
- Paxillus involutus
- TCA cycle
Status
Published
Research group
- Microbial Ecology
ISBN/ISSN/Other
- ISSN: 1574-6968