Influence of bioreactor scale and complex medium on probing control of glucose feeding in cultivations of recombinant strains of Escherichia coli
Author
Summary, in English
The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fedbatch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed regimes when transferring process technology from one bioreactor system to another.
Department/s
Publishing year
2007
Language
English
Pages
816-824
Publication/Series
Biotechnology and Bioengineering
Volume
97
Issue
4
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Control Engineering
Keywords
- probing control
- feeding strategy
- fed-batch
- fermentations
- industrial
- bioreactor
- complex medium
Status
Published
ISBN/ISSN/Other
- ISSN: 1097-0290