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Poly(beta-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1

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Author

Summary, in English

The moderate halophile Halomonas boliviensis, isolated from a Bolivian saline soil sample, was able to accumulate poly(P-hydroxybutyrate) (PHB) when grown under conditions of nutrient limitation and excess carbon source. The concentration of sodium chloride in the medium influenced the cell-growth, -size, and rate of PHB accumulation. Cultivation in shake flasks led to a PHB accumulation of about 54 wt.% with respect to cell dry weight at 4.5% (w/v) NaCl in a medium with butyric acid and sodium acetate as carbon sources. The production of PHB was substantially improved to a maximum value of 88 wt.% during cultivation under controlled conditions of pH and oxygen concentration in a fermentor. The use of glucose and sucrose, respectively, as carbon source could also lead to the production of PHB at an average level of 55 wt.%. (c) 2005 Elsevier Inc. All rights reserved.

Department/s

Publishing year

2006

Language

English

Pages

148-154

Publication/Series

Enzyme and Microbial Technology

Volume

38

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Industrial Biotechnology

Keywords

  • carbon source
  • poly(beta-hydroxybutyrate)
  • halonionas boliviensis
  • moderate halophile
  • (PHB) accumulation

Status

Published

ISBN/ISSN/Other

  • ISSN: 0141-0229