Specificity of the H-3-triolein assay for pancreatic lipase in blood plasma
Author
Summary, in English
The aim of this study was to investigate the specificity of the H-3-triolein assay and to investigate the recovery of highly purified pancreatic lipase and pancreatic lipase in the form of pure non-activated pig pancreatic juice. Blood plasma from pigs was analysed for pancreatic lipase activity using the H-3-triolein substrate assay, with a method specific for lipoprotein lipase and with a method specific for hepatic lipase. The recovery of pancreatic lipase from pancreatic juice was approximately 100%, while the recovery of highly purified pancreatic lipase in plasma or whole blood was found to be approximately 1%. Preparations of highly concentrated, purified lipoprotein lipase showed activity in the H-3-triolein assay designed for pancreatic lipase, but the activity did not exceed 1% of the activity of this enzyme measured in an assay specific for lipoprotein lipase (samples containing physiological levels of lipoprotein lipase did not show any activity in the assay). Hepatic lipase was not measurable under the conditions of the H-3-triolein assay. In conclusion, the H-3-triolein assay showed pronounced specificity for pancreatic lipase compared with lipoprotein lipase or hepatic lipase.
Department/s
Publishing year
2005
Language
English
Pages
1211-1214
Publication/Series
Clinical Chemistry and Laboratory Medicine
Volume
43
Issue
11
Document type
Journal article
Publisher
De Gruyter
Topic
- Clinical Laboratory Medicine
Keywords
- hepatic lipase
- lipoprotein lipase
- pancreatic lipase
Status
Published
Research group
- Appetite Regulation
ISBN/ISSN/Other
- ISSN: 1434-6621