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Specificity of the H-3-triolein assay for pancreatic lipase in blood plasma

Author

Summary, in English

The aim of this study was to investigate the specificity of the H-3-triolein assay and to investigate the recovery of highly purified pancreatic lipase and pancreatic lipase in the form of pure non-activated pig pancreatic juice. Blood plasma from pigs was analysed for pancreatic lipase activity using the H-3-triolein substrate assay, with a method specific for lipoprotein lipase and with a method specific for hepatic lipase. The recovery of pancreatic lipase from pancreatic juice was approximately 100%, while the recovery of highly purified pancreatic lipase in plasma or whole blood was found to be approximately 1%. Preparations of highly concentrated, purified lipoprotein lipase showed activity in the H-3-triolein assay designed for pancreatic lipase, but the activity did not exceed 1% of the activity of this enzyme measured in an assay specific for lipoprotein lipase (samples containing physiological levels of lipoprotein lipase did not show any activity in the assay). Hepatic lipase was not measurable under the conditions of the H-3-triolein assay. In conclusion, the H-3-triolein assay showed pronounced specificity for pancreatic lipase compared with lipoprotein lipase or hepatic lipase.

Publishing year

2005

Language

English

Pages

1211-1214

Publication/Series

Clinical Chemistry and Laboratory Medicine

Volume

43

Issue

11

Document type

Journal article

Publisher

De Gruyter

Topic

  • Clinical Laboratory Medicine

Keywords

  • hepatic lipase
  • lipoprotein lipase
  • pancreatic lipase

Status

Published

Research group

  • Appetite Regulation

ISBN/ISSN/Other

  • ISSN: 1434-6621