Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis
Author
Summary, in English
The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane.
Department/s
Publishing year
2008
Language
English
Pages
4660-4665
Publication/Series
Journal of Bacteriology
Volume
190
Issue
13
Document type
Journal article
Publisher
American Society for Microbiology
Topic
- Microbiology
- Biochemistry and Molecular Biology
Status
Published
ISBN/ISSN/Other
- ISSN: 0021-9193