Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.
Author
Summary, in English
Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.
Department/s
Publishing year
2008
Language
English
Pages
1330-1334
Publication/Series
FEBS Letters
Volume
582
Document type
Journal article
Publisher
Wiley-Blackwell
Topic
- Biochemistry and Molecular Biology
- Microbiology
Keywords
- CtaA
- Heme A synthesis
- Membrane protein evolution
- Cytochrome a
Status
Published
ISBN/ISSN/Other
- ISSN: 1873-3468