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Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.

Author

Summary, in English

Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.

Publishing year

2008

Language

English

Pages

1330-1334

Publication/Series

FEBS Letters

Volume

582

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Biochemistry and Molecular Biology
  • Microbiology

Keywords

  • CtaA
  • Heme A synthesis
  • Membrane protein evolution
  • Cytochrome a

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-3468