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Microfluidic components for protein characterization.

Author

Summary, in English

The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line digestion (12 s) and sample preparation of proteins, interfacing to capillary liquid chromatography (100 attomol sensitivity), and in-vial chemistry on femtomol amounts of sample are presented.

Publishing year

2001

Language

English

Pages

75-161

Publication/Series

Journal of Biotechnology

Volume

82

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering

Keywords

  • Miniaturization
  • Nanotechnology
  • Proteins : chemistry
  • Proteins : isolation & purification
  • Proteome
  • Silicon
  • Spectrometry
  • Mass
  • Matrix-Assisted Laser Desorption-Ionization : instrumentation
  • Equipment Design
  • Biotechnology : instrumentation
  • Bioreactors

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4863