Microfluidic components for protein characterization.
Author
Summary, in English
The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line digestion (12 s) and sample preparation of proteins, interfacing to capillary liquid chromatography (100 attomol sensitivity), and in-vial chemistry on femtomol amounts of sample are presented.
Department/s
Publishing year
2001
Language
English
Pages
75-161
Publication/Series
Journal of Biotechnology
Volume
82
Issue
2
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Medical Engineering
Keywords
- Miniaturization
- Nanotechnology
- Proteins : chemistry
- Proteins : isolation & purification
- Proteome
- Silicon
- Spectrometry
- Mass
- Matrix-Assisted Laser Desorption-Ionization : instrumentation
- Equipment Design
- Biotechnology : instrumentation
- Bioreactors
Status
Published
ISBN/ISSN/Other
- ISSN: 1873-4863