DNA fragmentation and ultrastructural changes of degenerating cells in atherosclerotic lesions and smooth muscle cells exposed to oxidized LDL in vitro
Author
Summary, in English
Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron microscopic analysis of cultured smooth muscle cells exposed to oxidized LDL in the absence of cytokines demonstrated cytoplasmic swelling and disruption of the plasma membrane, suggesting cell death by oncosis. Cells exposed to both oxidized LDL and cytokines were characterized by chromatin and cytoplasmic condensation compatible with cell death by apoptosis. These findings further support the notion that oxidized lipids play a role in plaque cell death.
Publishing year
1997
Language
English
Pages
2225-2231
Publication/Series
Arteriosclerosis, Thrombosis and Vascular Biology
Volume
17
Issue
10
Links
Document type
Journal article
Publisher
Lippincott Williams & Wilkins
Topic
- Cardiac and Cardiovascular Systems
Keywords
- apoptosis
- cell death
- oxidized LDL
- TNF{alpha}
- {gamma}IFN
Status
Published
ISBN/ISSN/Other
- ISSN: 1524-4636