A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor
Author
Summary, in English
A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.
Department/s
Publishing year
2005
Language
English
Pages
983-990
Publication/Series
Biotechnology Letters
Volume
27
Issue
14
Document type
Journal article
Publisher
Springer
Topic
- Industrial Biotechnology
- Control Engineering
Status
Published
ISBN/ISSN/Other
- ISSN: 1573-6776