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The quantitative proteome of a human cell line

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Author

  • Martin Beck
  • Alexander Schmidt
  • Johan Malmström
  • Manfred Claassen
  • Alessandro Ori
  • Anna Szymborska
  • Franz Herzog
  • Oliver Rinner
  • Jan Ellenberg
  • Ruedi Aebersold

Summary, in English

The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least similar to 10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution. Molecular Systems Biology 7: 549; published online 8 November 2011; doi:10.1038/msb.2011.82

Publishing year

2011

Language

English

Publication/Series

Molecular Systems Biology

Volume

7

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Immunology in the medical area

Keywords

  • mass spectrometry
  • protein abundance
  • proteomics

Status

Published

Research group

  • Infection Medicine Proteomics

ISBN/ISSN/Other

  • ISSN: 1744-4292