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Sodium-iodide symporter mediates iodide secretion in rat gastric mucosa in vitro.

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Author

Summary, in English

In vivo studies on rats have demonstrated that considerable amounts of iodide are transported from the bloodstream into the gastric lumen. The mechanisms for and functional significance of this transport are poorly understood. Active (driven by Na+/K+-ATPase) iodide transport into thyroid follicular cells is mediated by the sodium-iodide symporter (NIS), which is also abundantly expressed in gastric mucosa. We aimed to further investigate the iodide transport in gastric mucosa and the possible role of NIS in this transport process. Iodide transport in rat gastric mucosa was studied in vitro in an Ussing chamber system using 125I as a marker. The system allows measurements in both directions over a mucosal specimen. A considerable transport of iodide (from the serosal to the mucosal side) was established across the gastric mucosa, whereas in the opposite direction (mucosa to serosa), iodide transport was negligible. Sodium perchlorate (NaClO4), a competitive inhibitor of NIS, and ouabain, an inhibitor of the Na+/K+-ATPase, both attenuated gastric iodide transport from the serosal to the mucosal side. To investigate a possible neuroendocrine regulation of the iodide transport identified to occur from the serosal to the mucosal side of the stomach, thyroid-stimulating hormone (TSH), thyrotropin-releasing hormone (TRH), vasoactive intestinal peptide (VIP), histamine, or nitric oxide donor S-nitroso-N-acetyl-D,L-penicillamine (SNAP) was added. None of these substances influenced the iodide transport. We conclude that iodide is actively transported into the gastric lumen and that this transport is at least partly mediated by NIS. Additional investigations are needed to understand the regulation and significance of this transport.

Publishing year

2006

Language

English

Pages

277-281

Publication/Series

Experimental Biology and Medicine

Volume

231

Issue

3

Document type

Journal article

Publisher

SAGE Publications

Topic

  • Otorhinolaryngology
  • Cell and Molecular Biology

Keywords

  • gastric NIS
  • gastric iodide secretion
  • ouabain
  • sodium perchlorate
  • Ussing chamber

Status

Published

Research group

  • Laryngoesophagology, Allergy and Life Quality

ISBN/ISSN/Other

  • ISSN: 1535-3702