Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Summary, in English

Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase.

• Author: Henrik Stålbrand; Jon Lundqvist; Per Hägglund; Lars Anderson; Folke Tjerneld; A Jacobs; A Teleman; O Dahlman; Magnus Palm; Guido Zacchi; Alexandra Andersson
• Department/s: Biochemistry and Structural Biology; Division of Chemical Engineering
• Publishing year: 2004
• Language: English
• Pages: 66-78
• Publication/Series: Hemicelluloses. Science and Technology (ACS Symposium Series)
• Volume: 864
• Links: http://www.oup.com/us/catalog/general/subject/Chemistry/IndustrialChemistry/?view=usa&ci=0841238421
• Document type: Book chapter
• Publisher: The American Chemical Society (ACS)
• Topic: Chemical Engineering; Biological Sciences
• Keywords: DEGRADING ENZYMES; MICROWAVE IRRADIATION; BETA-MANNANASE; HEMICELLULOSES; PRETREATMENT; CHROMATOGRAPHY; LIGNOCELLULOSE; GLUCOMANNAN; POSITIONS; PINUS-SILVESTRIS L
• Status: Published
• ISBN/ISSN/Other: ISSN: 0097-6156; ISBN: 978-0-8412-3842-8; ISBN: 0-8412-3842-1