Different roles of spermine in glucocorticoid- and Fas-induced apoptosis
Author
Summary, in English
Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both thymocytes and Jurkat cells. Thus, spermine per se did not inhibit the caspases but rather their activation. The fact that spermine inhibited caspase activation only in the thymocytes implies that spermine inhibited dexamethasone-induced apoptosis upstream of caspase-9 activation.
Department/s
Publishing year
2001
Language
English
Pages
333-341
Publication/Series
Experimental Cell Research
Volume
266
Issue
2
Document type
Journal article
Publisher
Academic Press
Topic
- Cancer and Oncology
Keywords
- glucocorticoid
- apoptosis
- Fas death receptor
- thymocytes
- Jurkat cells
- sub-G1 peak
- cell cycle phase distribution
- polyamines
- caspases
- flow cytometry
Status
Published
ISBN/ISSN/Other
- ISSN: 1090-2422