Antigen-binding sites dominate the surface properties of IgG antibodies
Author
Summary, in English
A new technique, liquid-liquid partition chromatography in an aqueous polyethylene glycol-dextran two-phase system, was used to detect differences in surface properties of antibodies with different antigen-binding sites. Employing well-characterized monoclonal IgG antibodies and Fab and Fc fragments thereof as well as chimeric IgG antibodies we found a remarkable relationship between structure of the antibody combining site and chromatographic behaviour. The surface properties of the IgG antibodies were dominated by those of its antigen-binding regions. In addition, our results indicated that the constant parts of the IgGs form similar scaffoldings, on to which CDRs of variable shapes and sizes are interspaced and constitute the major dominant differences in exposed surface properties.
Department/s
Publishing year
1995
Language
English
Pages
819-827
Publication/Series
Molecular Immunology
Volume
32
Issue
11
Document type
Journal article
Publisher
Pergamon Press Ltd.
Topic
- Immunology in the medical area
Keywords
- Partition chromatography
- LLPC
- IgG antibodies
- antigen-binding sites
- surface properties
Status
Published
ISBN/ISSN/Other
- ISSN: 1872-9142