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Antigen-binding sites dominate the surface properties of IgG antibodies

Author

  • Christer Wingren
  • Ulla-Britt Hansson
  • Carl G.M Magnusson
  • Mats Ohlin

Summary, in English

A new technique, liquid-liquid partition chromatography in an aqueous polyethylene glycol-dextran two-phase system, was used to detect differences in surface properties of antibodies with different antigen-binding sites. Employing well-characterized monoclonal IgG antibodies and Fab and Fc fragments thereof as well as chimeric IgG antibodies we found a remarkable relationship between structure of the antibody combining site and chromatographic behaviour. The surface properties of the IgG antibodies were dominated by those of its antigen-binding regions. In addition, our results indicated that the constant parts of the IgGs form similar scaffoldings, on to which CDRs of variable shapes and sizes are interspaced and constitute the major dominant differences in exposed surface properties.

Publishing year

1995

Language

English

Pages

819-827

Publication/Series

Molecular Immunology

Volume

32

Issue

11

Document type

Journal article

Publisher

Pergamon Press Ltd.

Topic

  • Immunology in the medical area

Keywords

  • Partition chromatography
  • LLPC
  • IgG antibodies
  • antigen-binding sites
  • surface properties

Status

Published

ISBN/ISSN/Other

  • ISSN: 1872-9142