The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Distribution of respiratory mucin proteins in human nasal mucosa

På svenska

Author

  • DA Groneberg
  • C Peiser
  • QT Dinh
  • J Matthias
  • PR Eynott
  • W Heppt
  • Ingemar Carlstedt
  • C Witt
  • A Fischer
  • KF Chung

Summary, in English

Objectives/Hypothesis: The upper respiratory tract is involved in many acute and chronic respiratory tract diseases that present with the symptom of mucus hypersecretion. Mucin genes that encode for the backbone of glycoproteins contribute to the viscoelastic property of airway mucus. We examined the cellular expression and distribution of two major respiratory mucus-forming glycoproteins, MUC5AC and MUC5B, in normal human nasal tissues. Methods: Immunohistochemical analysis using polyclonal antibodies against the mucins MUC5AC and MUC5B was performed in normal human nasal tissues. Results: An abundant staining of submucosal mucus gland and epithelial goblet cells for MUC5B was found. hnmunohistochemical analysis of MUC5AC showed staining of surface epithelium goblet cells, whereas there was no staining of glandular cells. Comparison of the expression to lower airways revealed a similar pattern of expression of both mucins. Conclusions: The data in the present study demonstrated the localization of the two major respiratory mucin proteins in human nasal mucosa with a similar distribution of expression of MUC5AC and MUC5B in normal upper and lower airways. Mucin protein expression parallels that of mucin messenger RNA expression.

Publishing year

2003

Language

English

Pages

520-524

Publication/Series

Laryngoscope

Volume

113

Issue

3

Document type

Journal article

Publisher

Lippincott Williams & Wilkins

Topic

  • Cell and Molecular Biology

Keywords

  • immunohistochemistry
  • MUC5B
  • MUC5AC
  • mucin
  • nasal mucosa

Status

Published

ISBN/ISSN/Other

  • ISSN: 1531-4995