Intestinal bacteria and permeability during experimental acute pancreatitis in rats
Author
Summary, in English
Background: An increase in intestinal permeability and subsequent bacterial translocation has been demonstrated in
critical illness. Cellulose derivatives have in the past been shown to reduce gut leakage following liver resection.
Aims: The aim of the present study was to evaluate changes
in microbial counts in experimental acute pancreatitis and
the effect of pre-treatment with cellulose derivatives and
N-acetyl cysteine.
Subjects: 92 male Sprague Dawley rats.
Methods: Acute pancreatitis was induced by intraductal taurodeoxycholic acid infusion. Animals received oral pretreatment and were randomized to either sham operation
or the pancreatitis groups, with or without pre-treatment
with cellulose derivatives, the antioxidant or their combinations. Intestinal bacterial populations and permeability were evaluated using bacterial counts and Ussing chamber, respectively.
Results: The number of E. coli increased in the luminal content and ileal and colonic mucosa, but levels were restored to almost those seen in controls in all pre-treatment groups except for N-acetyl cysteine. When intestinal permeability was measured, none of the treatment groups showed significant differences compared to challenge, except for Nacetyl cysteine, which significantly increased permeability.
Conclusion: Pre-treatment with cellulose derivatives was
more efficient against disturbances in intestinal permeability and microbial populations than the antioxidant Nacetyl cysteine.
critical illness. Cellulose derivatives have in the past been shown to reduce gut leakage following liver resection.
Aims: The aim of the present study was to evaluate changes
in microbial counts in experimental acute pancreatitis and
the effect of pre-treatment with cellulose derivatives and
N-acetyl cysteine.
Subjects: 92 male Sprague Dawley rats.
Methods: Acute pancreatitis was induced by intraductal taurodeoxycholic acid infusion. Animals received oral pretreatment and were randomized to either sham operation
or the pancreatitis groups, with or without pre-treatment
with cellulose derivatives, the antioxidant or their combinations. Intestinal bacterial populations and permeability were evaluated using bacterial counts and Ussing chamber, respectively.
Results: The number of E. coli increased in the luminal content and ileal and colonic mucosa, but levels were restored to almost those seen in controls in all pre-treatment groups except for N-acetyl cysteine. When intestinal permeability was measured, none of the treatment groups showed significant differences compared to challenge, except for Nacetyl cysteine, which significantly increased permeability.
Conclusion: Pre-treatment with cellulose derivatives was
more efficient against disturbances in intestinal permeability and microbial populations than the antioxidant Nacetyl cysteine.
Publishing year
2006
Language
English
Pages
276-284
Publication/Series
Annals of Gastroenterology
Volume
19
Issue
3
Links
Document type
Journal article
Publisher
Hellenic Society of Gastroenterology
Topic
- Clinical Medicine
Keywords
- dietary fibres
- acute pancreatitis
- bacterial counts
- intestinal permeability
Status
Published
ISBN/ISSN/Other
- ISSN: 1108-7471