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Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine.

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Author

  • Tinkara Tinta
  • Louise Slot Christiansen
  • Anke Konrad
  • David A Liberles
  • Valentina Turk
  • Birgitte Munch-Petersen
  • Jure Piskur
  • Anders Ranegaard Clausen

Summary, in English

Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.

Publishing year

2012

Language

English

Pages

120-127

Publication/Series

FEMS Microbiology Letters

Volume

331

Issue

2

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Biological Sciences

Keywords

  • aquatic bacteria
  • 3H-thymidine incorporation
  • deoxyribonucleoside kinase
  • nucleoside salvage

Status

Published

ISBN/ISSN/Other

  • ISSN: 1574-6968