The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

A high throughput procedure for polycyclic aromatic hydrocarbons (PAHs) assayed for biodegradation in contaminated soils

På svenska

Author

Summary, in English

This study describes the development of a high throughput procedure for the extraction, purification, and quantification of all 16 polycyclic aromatic hydrocarbons (PAHs) on EPA's priority list, subject to biodegradation assays in a creosote-contaminated soil. We used various combinations of solvents, temperatures, ultrasonication, tumbling, saponification, vacuum centrifugation, adsorption chromatography, gas chromatography, and mass spectrometry to demonstrate that a) individual PAHs were quantified in the soil matrix with coefficient of variations varying between 5 and 28%, b) recovery from a spiked soil was 80% and more for most compounds but dependent on characteristics of the internal standard, c) up to 20 PAH analyses of soil samples can be processed per day by one person, with a reasonably low consumption of solvents, and d) from undetectable levels to >50% of PAHs were biodegraded in 45 days at 23 degreesC in soil samples that were contaminated with creosote more than 35 years ago.

Department/s

Publishing year

2001

Language

English

Pages

1-14

Publication/Series

International Journal of Environmental Analytical Chemistry

Volume

79

Issue

1

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Ecology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1029-0397