Derivation, characterization, and differentiation of human embryonic stem cells
Author
Summary, in English
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state.
Publishing year
2004
Language
English
Pages
367-376
Publication/Series
Stem Cells
Volume
22
Issue
3
Links
Document type
Journal article
Publisher
AlphaMed Press
Topic
- Cell and Molecular Biology
Keywords
- differentiation
- pluripotency
- human ES cell
- human blastocyst
- ICM
- subcloning
Status
Published
ISBN/ISSN/Other
- ISSN: 1549-4918