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Protein expression analysis: from "tip of the iceberg" to a global method.

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Author

Summary, in English

In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and new mass spectrometers, this emerging technology will be a rapid and highly effective alternative to gel-based methods with few of the latter's shortcomings.

Department/s

Publishing year

2001

Language

English

Pages

235-246

Publication/Series

Disease Markers

Volume

17

Issue

4

Document type

Journal article

Publisher

IOS Press

Topic

  • Medical Engineering

Keywords

  • Gel
  • Two-Dimensional : trends
  • Genome
  • Proteins : metabolism
  • Electrophoresis

Status

Published

ISBN/ISSN/Other

  • ISSN: 0278-0240