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Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine.

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Author

  • Stig Christoffersen
  • Immacolata Serra
  • M Terreni
  • Jure Piskur

Summary, in English

Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.

Publishing year

2010

Language

English

Pages

445-448

Publication/Series

Nucleosides, Nucleotides & Nucleic Acids

Volume

29

Issue

4-6

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Biological Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 1525-7770