MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.

Summary, in English

As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD < 25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.

• Author: Melinda Rezeli; Ákos Végvári; Jan Ottervald; Tomas Olsson; Thomas Laurell; György Marko-Varga
• Department/s: Department of Biomedical Engineering; Departments at LTH; MultiPark: Multidisciplinary research focused on Parkinson´s disease
• Publishing year: 2011
• Language: English
• Pages: 211-220
• Publication/Series: Journal of Proteomics
• Volume: 75
• Issue: 1
• Full text: - 950 kB; Download statistics
• Links: http://dx.doi.org/10.1016/j.jprot.2011.05.042
• Document type: Journal article
• Publisher: Elsevier
• Topic: Medical Engineering
• Keywords: linear ion trap; multiple sclerosis; selective reaction monitoring
• Status: Published
• ISBN/ISSN/Other: ISSN: 1874-3919