The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis

På svenska

Author

  • Georg Wieczorek
  • Christine Steinhoff
  • Ralph Schulz
  • Marina Scheller
  • Martin Vingron
  • H-Hilger Ropers
  • Ulrike Nuber

Summary, in English

Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.

Publishing year

2003

Language

English

Pages

227-237

Publication/Series

Cell and Tissue Research

Volume

311

Issue

2

Document type

Journal article

Publisher

Springer

Topic

  • Cell Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1432-0878