The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.

På svenska

Author

Summary, in English

The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene.

Publishing year

2002

Language

English

Pages

261-270

Publication/Series

Journal of Immunology

Volume

169

Issue

1

Document type

Journal article

Publisher

American Association of Immunologists

Topic

  • Immunology in the medical area

Keywords

  • Transcription Factors : physiology
  • Trans-Activators : biosynthesis
  • Inbred BALB C
  • Mice
  • Cells
  • Nuclear Proteins : metabolism
  • Organ Specificity : genetics
  • Protein Binding : immunology
  • Organ Specificity : immunology
  • Trans-Activators : genetics
  • E-Box Elements : genetics
  • E-Box Elements : immunology
  • Exons : genetics
  • Exons : immunology
  • Hela Cells
  • Helix-Loop-Helix Motifs : genetics
  • Helix-Loop-Helix Motifs : immunology
  • Human
  • Molecular Sequence Data
  • Nuclear Proteins : physiology
  • Promoter Regions (Genetics) : immunology
  • Protein Binding : genetics
  • Support
  • Non-U.S. Gov't
  • Trans-Activators : chemistry
  • Trans-Activators : physiology
  • Nuclear Proteins : genetics
  • Inbred C57BL
  • DNA-Binding Proteins : physiology
  • DNA-Binding Proteins : genetics
  • DNA-Binding Proteins : chemistry
  • DNA-Binding Proteins : biosynthesis
  • Molecular
  • Cloning
  • Cultured
  • 5' Untranslated Regions : genetics
  • 5' Untranslated Regions : immunology
  • Amino Acid Sequence
  • Animal
  • B-Lymphocytes : immunology
  • B-Lymphocytes : metabolism
  • Base Composition
  • Base Sequence

Status

Published

ISBN/ISSN/Other

  • ISSN: 1550-6606