Derivation of a xeno-free human ES cell line.

Summary, in English

Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-contaminated hESCs into patients, such as an increased risk of graft rejection [STEM CELLS 2006;24:221229] and the potential transfer of nonhuman pathogens, make existing hESC lines unsuitable for clinical applications. To avoid xeno-contamination during derivation and culture of hESCs, we first developed a xeno-free medium supplemented with human serum, which supports long-term (> 50 passages) culture of hESCs in an undifferentiated state. To enable derivation of new xeno-free hESCs, we also established xeno-free human foreskin fibroblast feeders and replaced immunosurgery, which involves the use of guinea pig complement, with a modified animal-product-free derivation procedure. Here, we report the establishment and characterization (> 20 passages) of a xeno-free pluripotent diploid normal hESC line, SA611.

• Author: Catharina Ellerström; Raimund Strehl; Karina Moya; Katarina Andersson; Christina Bergh; Kersti Lundin; Johan Hyllner; Henrik Semb
• Department/s: Stem Cell Center
• Publishing year: 2006
• Language: English
• Pages: 2170-2176
• Publication/Series: Stem Cells
• Volume: 24
• Issue: 10
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16741223&dopt=Abstract; http://dx.doi.org/10.1634/stemcells.2006-0130
• Document type: Journal article
• Publisher: AlphaMed Press
• Topic: Cell and Molecular Biology
• Keywords: human feeders; human serum; clinical; therapies; human embryonic stem cell
• Status: Published
• ISBN/ISSN/Other: ISSN: 1549-4918