The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Picodroplet-deposition of enzymes on functionalized self-assembled monolayers as a basis for miniaturized multi-sensor structures

På svenska

Author

  • M Mosbach
  • H. Zimmermann
  • Thomas Laurell
  • J. Nilsson
  • Elisabeth Csöregi
  • W. Schuhmann

Summary, in English

We are reporting on a novel approach for structured immobilisation of enzymes on gold surfaces modified with monolayers of functionalised alkylthiols. The formation of enzyme spots is achieved by shooting very small volumes of an appropriate enzyme solution (down to 100 pl) onto a thiol-monolayer modified gold surface using a micro-dispenser. Formation of enzyme patterns is obtained by moving the micro-dispenser relative to the modified gold surface using a micro-positioning device. Enzyme spots with typical lateral dimensions of 100 ml are obtained, but also, more complex structures, e.g. lines or meander structures, can be achieved by multiple droplets dispensed during the concomitant movement of the micro-dispenser. The first enzyme layer on top of the functionalised thiol-monolayer is subsequently covalently immobilised using either carbodiimide activation of carboxilic headgroups at the enzyme or via already introduced activated ester functions at the monolayer. Immobilised enzyme activities of glucose oxidase and lactate oxidase patterns have been characterised by means of scanning electrochemical microscopy. The product of the enzyme-catalysed reaction, H2O2, is detected with an micro-electrode in the presence of either or both substrates, glucose and lactate, leading to a visualisation of the corresponding enzyme pattern and the lateral enzymatic activity. (C) 2001 Elsevier Science B.V. All rights reserved.

Publishing year

2001

Language

English

Pages

827-837

Publication/Series

Biosensors & Bioelectronics

Volume

16

Issue

9-12

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering
  • Industrial Biotechnology

Keywords

  • microdispenser
  • microstructure
  • enzymes
  • scanning electrochemical
  • microscopy
  • SCANNING ELECTROCHEMICAL MICROSCOPY
  • MODIFIED GOLD ELECTRODE
  • CAPILLARY-ELECTROPHORESIS
  • AMPEROMETRIC BIOSENSORS
  • GLUCOSE-OXIDASE
  • REDOX ENZYMES
  • SURFACES
  • IMMOBILIZATION
  • POLYPYRROLE
  • FABRICATION

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4235