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Preparation, crystallization and preliminary X-ray analysis of protein YtlP from Bacillus subtilis

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Author

Summary, in English

Bacillus subtilis YtlP is a protein that is predicted to belong to the bacterial and archael 2'-5' RNA-ligase family. It contains 183 residues and two copies of the HXTX sequence motif conserved among proteins belonging to this family. In order to determine the structure of YtlP and to compare it with the paralogue YjcG and identified 2'-5' RNA ligases, the gene ytlP was amplified from B. subtilis genomic DNA and cloned into expression vector pET-21a. The soluble protein was produced in Escherichia coli, purified to homogeneity and crystals suitable for X-ray analysis were obtained. The crystal diffracted to 2.0 angstrom and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.16, b = 48.54, c = 105.75 angstrom.

Publishing year

2006

Language

English

Pages

967-969

Publication/Series

Acta Crystallographica. Section F: Structural Biology and Crystallization Communications

Volume

62

Issue

10

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Microbiology
  • Biochemistry and Molecular Biology

Keywords

  • 2′-5′ RNA-ligase family

Status

Published

ISBN/ISSN/Other

  • ISSN: 2053-230X