Quantification of Membrane Proteins Using Nonspecific Protease Digestions
Author
Summary, in English
We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.
Publishing year
2009
Language
English
Pages
5666-5673
Publication/Series
Journal of Proteome Research
Volume
8
Issue
12
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Immunology in the medical area
Keywords
- membrane proteins
- N-terminal labeling
- quantification
- relative
- proteinase K
- Bacillus subtilis
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-3893