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Capturing ion exchanger-bound infectious pancreatic necrosis virus - design and application for large volume water samples

Author

Summary, in English

A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 1 water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide eel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total concentration factor ranged between 150000 and 250000 times, depending on the requirements and the methods of choice.

Publishing year

2003

Language

English

Pages

173-178

Publication/Series

Journal of Virological Methods

Volume

110

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • ion exchanger
  • IPNV
  • virus concentration
  • filtration device

Status

Published

ISBN/ISSN/Other

  • ISSN: 1879-0984