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Vital staining of fungi in pure cultures and in soil with fluorescein diacetate

På svenska

Author

Summary, in English

A method for vital staining of fungal mycelium with fluorescein diacetate (FDA) is described. In experiments with pure cultures, good correlation was obtained between relative staining efficiency, growth rate, and respiration. FDA thus appears to be a true vital stain, in that it stains only metabolically active mycelia.



In fresh soil suspensions stained with FDA, brightly fluorescent hyphae and portions of hyphae were observed. The applicability of the method for measurement of active mycelium in the soil is discussed.

Publishing year

1977

Language

English

Pages

59-63

Publication/Series

Soil Biology & Biochemistry

Volume

9

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Status

Published

Research group

  • Microbial Ecology

ISBN/ISSN/Other

  • ISSN: 0038-0717