Flow-through picoliter dispenser: A new approach for solvent elimination in FT-IR spectroscopy

Summary, in English

A new interface for FT-IR analysis of liquid samples on the basis of solvent elimination is presented. The approach is based on a piezoactuated flow-through microdispenser, a device built of two microstructured silicon wafers designed for micro-liquid handling. It could be verified during preliminary studies using a sequential injection (SI) system for automated liquid handling that the flow-through microdispenser as a possible interface for flow system-FT-IR analysis has the capability of meeting the demands of hyphenated miniaturized liquid handling systems (e.g., mu-HPLC, microhigh performance liquid chromatography), as it successfully provides highly stable, reliable and reproducible operating conditions for liquid handling in the picoliter range. Moreover, an increase in sensitivity for FT-IR measurements could be achieved, lowering the mass detection limit of sugars (such as the investigated sucrose) to 53 picograms. As is demonstrated on the example of an HPLC separation of a mixture of glucose and fructose, interfacing LC systems to FT-IR using a piezoactuated flow-through microdispenser is a feasible and promising approach.

• Author: M Haberkorn; J Frank; M Harasek; Johan Nilsson; Thomas Laurell; B Lendl
• Department/s: Department of Biomedical Engineering
• Publishing year: 2002
• Language: English
• Pages: 902-908
• Publication/Series: Applied Spectroscopy
• Volume: 56
• Issue: 7
• Links: http://dx.doi.org/10.1366/000370202760171581
• Document type: Journal article
• Publisher: Society for Applied Spectroscopy
• Topic: Medical Engineering
• Keywords: micro-liquid handling; FT-IR; infrared spectroscopy; Fourier transform; flow-through microdispenser; solvent elimination
• Status: Published
• ISBN/ISSN/Other: ISSN: 1943-3530