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Long-term maintenance of hematopoietic stem cells does not require contact with embryo-derived stromal cells in cocultures

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Author

  • Robert A J Oostendorp
  • Catherine Robin
  • Christine Steinhoff
  • Stefanie Marz
  • Rosalinde Brauer
  • Ulrike Nuber
  • Elaine A Dzierzak
  • Christian Peschel

Summary, in English

We recently established that two midgestation-derived stromal clones--UG26-1B6, urogenital ridge-derived, and EL08-1D2, embryonic liver-derived--support the maintenance of murine adult bone marrow and human cord blood hematopoietic repopulating stem cells (HSCs). In this study, we investigate whether direct HSC-stroma contact is required for this stem cell maintenance. Adult bone marrow ckit+ Ly-6C- side population (K6-SP) cells and stromal cells were cocultured under contact or noncontact conditions. These experiments showed that HSCs were maintained for at least 4 weeks in culture and that direct contact between HSCs and stromal cells was not required. To find out which factors might be involved in HSC maintenance, we compared the gene expression profile of EL08-1D2 and UG26-1B6 with four HSC-nonsupportive clones. We found that EL08-1D2 and UG26-1B6 both expressed 21 genes at a higher level, including the putative secreted factors fibroblast growth factor-7, insulin-like growth factor-binding proteins 3 and 4, pleiotrophin, pentaxin-related, and thrombospondin 2, whereas 11 genes, including GPX-3 and HSP27, were expressed at a lower level. In summary, we show for the first time long-term maintenance of adult bone marrow HSCs in stroma noncontact cultures and identify some secreted molecules that may be involved in this support.

Publishing year

2005

Language

English

Pages

842-851

Publication/Series

Stem Cells

Volume

23

Issue

6

Document type

Journal article

Publisher

AlphaMed Press

Topic

  • Cell and Molecular Biology

Keywords

  • Hematopoiesis
  • Hematopoietic stem cells
  • AGM
  • Transwell
  • Microenvironment

Status

Published

ISBN/ISSN/Other

  • ISSN: 1549-4918