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Design, viualisation and utilisation of enzyme microstructures built on solid surfaces

Author

Summary, in English

Abstract in Undetermined
Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.) and active placement methods (electrospraying, microdispensing, etc.). Some key visualization techniques of enzyme microstructures (fluorescence microscopy, scanning electrochemical microscopy, etc.) are also mentioned together with examples of their applications or application possibilities.

Publishing year

2002

Language

English

Pages

245-266

Publication/Series

Reviews in Analytical Chemistry

Volume

21

Issue

4

Document type

Journal article

Publisher

Freund Publishing House Ltd

Topic

  • Analytical Chemistry
  • Medical Engineering

Keywords

  • scanning electrochemical microscopy
  • enzyme microstructures
  • microdispenser

Status

Published

ISBN/ISSN/Other

  • ISSN: 0048-752X