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MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells.

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Author

  • Julia Davies
  • Sara Kirkham
  • Naila Svitacheva
  • David J Thornton
  • Ingemar Carlstedt

Summary, in English

The gel-forming MUC5AC and MUC5B mucins have been identified as major components of human airway mucus but it is not known whether additional mucin species, possibly with other functions, are also present. MUC16 mucin is a well-known serum marker for ovarian cancer, but the molecule has also been found on the ocular surface and in cervical secretions suggesting that it may play a role on the normal mucosal surface. In this investigation, the LUM16-2 antiserum (raised against a sequence in the N-terminal repeat domain) recognized MUC16 in goblet and submucosal gland mucous cells as well as on the epithelial surface of human tracheal tissue suggesting that the mucin originates from secretory cells. MUC16 mucin was present in ‘normal’ respiratory tract mucus as well as in secretions from normal human bronchial epithelial (NHBE) cells. MUC16 from NHBE cells was a high-molecular-mass, monomeric mucin which gave rise to large glycopeptides after proteolysis. N- and C-terminal fragments of the molecule were separated on gel electrophoresis showing that the MUC16 apoprotein undergoes a cleavage between these domains, possibly in the SEA domain as demonstrated for other transmembrane mucins; MUC1 and MUC3. After metabolic labeling of NHBE cells, most of the secreted monomeric, high-molecular-mass [35S]sulphate-labelled molecules were immunoprecipitated with the OC125 antibody indicating that MUC16 is the major [35S]sulphate-labelled mucin in NHBE cell secretions.

Publishing year

2007

Language

English

Pages

1943-1954

Publication/Series

International Journal of Biochemistry & Cell Biology

Volume

39

Issue

10

Document type

Journal article

Publisher

Elsevier

Topic

  • Cell and Molecular Biology

Keywords

  • epithelium
  • MUC16
  • submucosal
  • glands
  • bronchial
  • epithelial

Status

Published

ISBN/ISSN/Other

  • ISSN: 1878-5875